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The eight playable characters include:
- Akira Yuki: A Kung Fu teacher from Japan, Akira Yuki fights using Bajiquan.
- Jacky Bryant: The older brother of Sarah Bryant and a race car driver from San Francisco, California, Jacky Bryant fights using Jeet Kune Do.
- Sarah Bryant: The younger sister of Jacky Bryant and a college student from San Francisco, California, Sarah Bryant also fights using Jeet Kune Do.
- Lau Chan: The father of Pai Chan and a cook from China, Lau Chan fights using Koen-Ken.
- Pai Chan: The daughter of Lau Chan and a martial arts movie star from Hong Kong, Pai Chan fights using Mizongquan.
- Kage-Maru: A ninja from Japan, Kage-Maru fights using Ninjutsu.
- Wolf Hawkfield: A professional wrestler from Canada, Wolf Hawkfield fights using Professional Wrestling.
- Jeffry McWild: A fisherman from Australia, Jeffry McWild fights using Pancratium.