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Duke Nuke 3D uses the following keys by default; they can be modified in the game's options screen.
- ↑ Forward
- ↓ Back
- ← Turn left
- → Turn right
- A Jump
- Z Crouch
- , Strafe left
- . Strafe right
- alt + ← → Strafe
- backspace Turn around
- space Open/action
- shift Run
- caps lock Autorun
- ctrl Fire weapon
- 1 - 0 : select weapon 1-0
- ' Next weapon
- ; Previous weapon
- ` (bkwd apostrophe) Quick kick, a.k.a. "Mighty Foot" (some Windows ports use this to open a command console; position of Mighty Foot varies)
- [ ] : Inventory left/right
- enter : Use item
- home Momentary look up
- end Momentary look down
- insert Momentary look left
- delete Momentary look right
- pgup Aim up
- pgdn Aim down
- 5 (on keypad) Center aim
- tab Map
- F Map follow mode
- H Holoduke
- I Crosshair on/off
- J Jetpack on/off
- K Show co-op view (net game)
- M Portable medkit
- N Night-vision on/off
- T Send message (net game)
- U Toggle mouse aiming
- W Show opponent's weapon (net game)
- scroll lock Holster weapon
- F1 Key help
- F2 Save
- F3 Load
- F4 Sound setup
- F5 Music
- shift + F5 Change music
- F6 Quicksave
- F7 First/third person view
- F8 Toggle messages
- F9 Quickload
- F10 Quit
- F11 Gamma adjust
- F12 Screenshot
- + Increase screen size
- - Decrease screen size
- pause Pause