The following is a list of major characters in the story. These do not include all of the bosses and enemies, although some of these characters can be categorized as such.
Protagonists
Pit
Our Hero.
Palutena
The wise mentor, and giver of the power of flight.
Underworld Army
Medusa
The main antagonist.
Dark Lord Gaol
A warlord who serves Medusa.
Hewdraw
One of Medusa's generals.
Pandora
The Goddess of Deceit, and one of Medusa's generals.
Thanatos
The God of Death, and Medusa's second in command
Hades
The manipulator who revived Medusa, and the game's true antagonist.
Forces of Nature
Viridi
The Goddess of Nature, who hates humanity and the Underworld alike.
Arlon the Serene
One of Viridi's commanders, and the guardian of the Lunar Sanctum.
Phosphora
One of Viridi's commanders, and a user of lightning attacks.
Unaffiliated
Magnus
A human mercenary, who happens to fight the Underworld Army.
Dark Pit/Pittoo
A clone of Pit, who only fights for his own pleasure.
Poseidon
The God of the Oceans
Pyrrhon
God of the Sun, and a large ham in a pantheon of ham.
The Chariot Master
Guardian of the Lightning Chariot.
Dyntos
God of the Forge, and the creator of great weapons.