- Hamtaro: A white and orange male ham-ham. He works hard to help his friends.
- Bijou: A white female ham-ham that wears two blue bows.
- Cappy: A white and brown male ham-ham that wears a green cap. He loves hats and is rarely seen without one.
- Boss: An older tan and brown spotted field ham-ham that is somewhat of a leader to the others.
- Oxnard: A white and grey spotted male ham-ham. He is Hamtaro’s best friend and somewhat of a worrywart.
- Pashmina: A white and tan female ham-ham that wears a pink scarf. She is rarely seen without her sister Penelope.
- Penelope: Pahsmina's little sister. Even though she can't talk yet Pashmina can understand her.
- Daisy: The yellow Rainbow Girl.
- Ivy: The green Rainbow Girl.
- Rosy: The magenta Rainbow Girl.
A team straight out of the jungle. All of them wearing animal like costumes from the jungle.
- Leo: A ham-ham dressed like a lion.
- Bunny: A ham-ham dressed like a pink bunny.
- Stripes: A ham-ham dressed like a zebra.
- Warts: A ham-ham dressed like a green frog.
A pirate themed team. All of the crew wear red and white striped outfits.
- Captain Hamstern: