The father of Ryu Hayabusa, Joe has returned from training in distant lands to resume his duties as the leader of the Hayabusa Ninja Clan, only to be injured in one of his many battles against the forces of evil. He lays in rest at his home in the Castle of the Dragon, unaware that the evil shadow of the Black Spider Clan slowly descends upon his village.
Overlord of the Black Spider Ninja Clan, Genshin's lust for power is matched only by his hatred of Ryu Hayabusa and his bloodline. Hoping to gain an upper hand in the struggle for Ninja superiority, Genshin enters into an uneasy allegiance with the Fiends. He will stop at nothing to destroy Ryu and put an end to this centuries-old feud once and for all.
She apparently works for the CIA and is looking for Ryu to stop the Black Spider Clan from obtaining the Demon Statue, possessed by the Hayabusa Ninja Clan, in order to resurrect the Arch-Fiend.
Ruler of Blood and Queen of the Greater Fiends, Elizébet is tasked with retrieving an important ancient artifact from the ancestral village of Ryu Hayabusa, setting into motion a sequence of events that will threaten the very existence of the human race.Her name and the fact that she is the Ruler of Blood is probably derived from the murderous Elizabeth Bathory, also known as the Blood Queen.
Ruler of Storms and one of the Four Greater Fiends, Volf is a vicious warrior who seeks an opponent worthy of himself. His Lycanthrope underlings have taken over the canal-lined streets of the tranquil Aqua Capital as he sits in his throne in the castle above.
A legendary blacksmith who first appeared in Ninja Gaiden (Xbox), made a cameo in Dead or Alive 4, and then in Ninja Gaiden Dragon Sword. In the game, it is shown that he is an accomplished swordsman as well.
One of the Four Greater Fiends, and Ruler of Lightning, his hatred of humankind knows no bounds. He has taken the city of New York in his evil grasp and waits perched upon the Statue of Liberty, challenging Ryu Hayabusa to come and face him.
One of the four Greater Fiends and Ruler of Flame, Zedonius has nothing but contempt for human life. He has descended upon the capital of a once-great military superpower, threatening all humankind with an ultimatum: submit to the will of the Fiends or be burned by infernal flames. Ryu must ascend the city's clocktower to put an end to his fearsome reign. He claims that he bestowed fire upon the humans centuries ago. He has a habit of calling humans "apes" and "monkeys".