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Koopa Kid, Toad, and Boo are not playable in Story Mode. Koopa Kid serves as the opponent for all stages; Toad is the player's CPU partner for a 2 vs. 2 minigame (via the VS. Spaces) if there are two Koopa Kids remaining. The only playable characters in Story Mode are Mario, Luigi, Peach, Daisy, Wario, Waluigi, and Yoshi.
Characters
- Mario
- Luigi
- Peach
- Daisy
- Wario
- Waluigi
- Yoshi
- Toad
- Boo
- Koopa Kid
Default Partners
- Mario and Luigi
- Peach and Daisy
- Wario and Waluigi
- Yoshi and Toad
- Boo and Koopa Kid