Standard
Light Weight
- Toad
- Koopa Troopa
- Baby Mario
- Baby Peach
Middle Weight
- Mario
- Luigi
- Peach
- Yoshi
Heavy Weight
- Wario
- Donkey Kong
- Bowser
- Waluigi
Unlockables
Warning, Spoilers Ahead
Light Weight
- Baby Luigi
How To Unlock: Unlock 8 Expert Staff Ghost Data on Time Trials.
- Baby Daisy
How To Unlock: Attain at least 1 Star Rank for all 50cc Nitro Grand Prix Cups.
- Dry Bones
How To Unlock: Attain a Gold Trophy in the 100cc Leaf Cup.
- Toadette
How To Unlock: Play on all 32 courses in Time Trials.
Middle Weight
- Daisy
How To Unlock: Attain a Gold Trophy in 150cc Special Cup.
- Diddy Kong
How To Unlock: Attain Gold Trophies in all 50cc Cups.
- Birdo
How To Unlock: a) Beat 250 people while playing online.
b) Play on 16 courses in Time Trials.
- Bowser Jr.
How To Unlock: Attain at least 1 Star Rank for all 100cc Retro Cups.
Heavy Weight
- Boo
How To Unlock: Attain a Gold Trophy in 50cc Star Cup.
- Rosalina
How To Unlock: a) With a Super Mario Galaxy save on your Wii, attain Gold Trophies in all of at least one series of Cups.
b) Attain at least 1 Star Rank for all Mirror Retro Grand Prix Cups.
- Funky Kong
How To Unlock: Unlock 4 Expert Staff Ghost Data in Time Trials.
- Dry Bowser
How To Unlock: Attain at least 1 Star Rank in all 150cc Nitro Grand Prix Cups.
Miscellaneous
- Mii (Outfit A)
How To Unlock: Attain a Gold Trophy at 100cc Special Cup.
- Mii (Outfit B)
How To Unlock: Unlock all Expert Staff Ghost Data in Time Trials.