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In Borderlands 2, The challenge system is back. There are five tiers for each one. When you complete one tier, you move onto the next. There are 720 different challenges in all.

Challenge Objective Number required for Level
1 2 3 4 5
Enemies
Pod Pew Pew
(Unlocked upon entering Tundra Express)
Kill varkid pods before they hatch. 10 25 45 70 100
Pest Control
(Unlocked upon entering Frostburn Canyon)
Kill spiderants. 10 25 75 150 300
Little Person, Big Pain Kill midgets. 10 25 75 150 300
Die in the Friendly Skies
(Unlocked upon entering The Dust)
Kill buzzards. 10 25 45 70 100
Another Bug Hunt
(Unlocked upon entering Tundra Express)
Kill varkids. 10 25 75 150 300
Skags to Riches
(Unlocked upon entering Three Horns - Divide)
Kill skags. 10 25 75 150 300
Marauder? I Hardly Know 'Er Kill marauders. 20 100 500 1000 1500
You Dirty Rat Kill rats. 10 25 75 150 300
Mama's Boys psychos. 50 100 150 300 500
You (No)Mad, Bro? Kill nomads. 10 25 75 150 300
Just a Moment of Your Time... Kill surveyors. 10 25 75 150 300
Hurly Burly Shoot bullymong-tossed projectiles out of midair. 10 25 50 125 250
Paingineering Hyperion personnel. 10 25 75 150 300
Crystals are a Girl's Best Friend
(Unlocked upon entering The Fridge)
Kill crystalisks. 10 25 50 80 120
You're One Ugly Mother...
(Unlocked upon entering The Highlands - Outwash)
stalkers. 10 25 75 150 300
Tentacle Obsession
(Unlocked upon entering The Highlands - Outwash)
Kill threshers. 10 25 75 150 300
Cruising for a Bruising bruisers. 10 25 75 150 300
WHY SO MUCH HURT?! Kill goliaths. 10 25 50 80 120
Bully the Bullies Kill bullymongs. 25 50 150 300 750
Load and Lock Kill loaders. 20 100 500 1000 1500
Constructor Destructor Kill constructors. 5 12 20 30 50
Short-Chained midgets from shields. 1 5 15 30 50
Elemental Damage|Elemental
Cowering Inferno Ignite enemies. 25 100 400 1000 2000
Acid Trip Kill enemies with corrosive damage. 20 75 250 600 1000
Slag-Licked Deal bonus damage to Slagged enemies. 5k 25k 150k 1M 5M
Say "Watt" Again Deal Shock damage. 5k 20k 100k 500k 1M
Corroderate Deal corrosive damage. 2.5k 20k 100k 500k 1M
I Just Want to Set the World on Fire burn damage. 2.5k 20k 100k 500k 1M
Boom Kill enemies with Explosive damage. 20 75 250 600 1000
Loot
Purple Reign Loot or purchase purple items. 2 4 7 12 20
I Like My Treasure Rare Loot or purchase blue items. 5 12 20 30 45
It's Not Easy Looting Green Loot or purchase green items. 20 50 75 125 200
Another Man's Treasure Loot or purchase white items. 50 125 250 400 600
Gun Runner Pick up or purchase Weapons. 10 25 150 300 750
Open Pandora's Boxes lootable chests, lockers, and other objects. 50 250 750 1500 2500
Nothing Rhymes with Orange Loot or purchase orange items. 1 3 6 10 15
The Call of Booty treasure chests. 5 25 50 125 250
Money and Trading
Dolla Dolla Bills, Y'all Collect Money from cash drops. 5k 25k 125k 500k 1M
Psst, Hey Buddy... Trade with other players. 1 5 15 30 50
For the Hoard! Save a lot of money. 10k 50k 250k 1M 3M
Wholesale Sell items to vending machines. 10 25 150 300 750
Limited-Time Offer Buy Items of the Day. 1 5 15 30 50
Whaddaya Buyin'? Purchase items with Eridium. 2 5 9 14 20
Vehicle
Hit-and-Fun Kill enemies by ramming them with a vehicle. 5 10 50 100 200
Passive Aggressive Kill enemies while riding as a passenger (not a gunner) in a vehicle. 1 10 50 100 200
Turret Syndrome Kill enemies using a turret or vehicle-mounted weapon. 10 25 150 300 750
...One Van Leaves Kill vehicles while in a vehicle. 5 10 50 100 200
Blue Sparks Kill enemies by power-sliding over them in a vehicle. 5 15 30 50 75
Health and Recovery
Acid Trip Level 1) Get Second Winds by killing enemies with corrosive DoT (damage over time). 1 5 15 30 50
Cowering Inferno Level 1) Get Second Winds by killing enemies with burn DoT (damage over time). 1 5 15 30 50
Say "Watt" Again Level 1) Get Second Winds by killing enemies with electrocute DoT (damage over time). 1 5 15 30 50
I'll Just Help Myself enemy. 5 10 50 100 200
Heal Plz Recover Health. 1k 25k 150k 1M 5M
This is No Time for Lazy! Revive a co-op partner. 5 10 50 100 200
Badass Bingo Get Second Winds by killing a badass enemy. 1 5 15 30 50
Grenades
EXPLOOOOOSIONS! Kill enemies with MIRV Grenades. 10 25 75 150 300
Health Vampire Transfusion grenades. 10 25 75 150 300
Woah, Black Betty Kill enemies with Bouncing Betty Grenades. 10 25 75 150 300
Chemical Sprayer Area of Effect grenades. 10 25 75 150 300
Singled Out Kill enemies with Singularity Grenades. 10 25 75 150 300
Pull the Pin Kill enemies with grenades. 10 25 150 300 750
Shields
Amp It Up Kill enemies while buffed by an Amplify Shield. 5 10 50 100 200
Super Novas Kill enemies with a Nova Shield burst. 5 10 50 100 200
Roid Rage Kill enemies while buffed by a "Maylay" shield. 5 10 50 100 200
Ammo Eater Absorption Shield. 20 75 250 600 1000
Game of Thorns Kill enemies with reflected damage from a Spike Shield. 5 10 50 100 200
Rocket Launcher
Rocket and Roll Kill enemies with rocket launchers. 10 50 100 250 500
Splish Splash
(Unlocked by completing Rocket and Roll Level 1)
Kill enemies with rocket launcher splash damage. 5 10 50 100 200
Shield Basher
(Unlocked by completing Splish Splash Level 1)
Kill shielded enemies with one rocket each. 5 15 35 75 125
Catch-a-Rocket!
(Unlocked by completing Splish Splash Level 5)
Kill enemies with direct rocket hits. 5 10 50 100 200
Gone with the Second Wind Get Second Winds with rocket launchers. 2 5 15 30 50
Sky Rockets in Flight...
(Unlocked by completing Gone With the Second Wind Level 1)
Kill enemies from long range with rocket launchers. 25 100 400 1000 2000
Sniper Rifle
Longshot Kill enemies with sniper rifles. 20 100 500 2500 5000
Eviscerated
(Unlocked by completing Longshot Level 1)
Kill shielded enemies with one shot using sniper rifles. 5 15 35 75 125
No Scope, No Problem
(Unlocked by completing Longshot Level 1)
Kill enemies with sniper rifles without using ironsights. 5 10 50 100 200
Surprise!
(Unlocked by completing No Scope, No Problem Level 1)
Kill unaware enemies with sniper rifles. 5 10 50 100 200
Longshot Headshot critical hits with sniper rifles. 25 100 400 1000 2000
Snipe Hunting
(Unlocked by completing Longshot Headshot Level 1)
critical hits using sniper rifles. 10 25 75 150 300
Leaf on the Second Wind Get Second Winds with sniper rifles. 2 5 15 30 50
Assault Rifle
Aggravated Assault Kill enemies with assault rifles. 25 100 400 1000 2000
Crouching Tiger, Hidden Assault Rifle
(Unlocked by completing Aggravated Assault Level 1)
Kill enemies with assault rifles while crouched. 25 75 400 1600 3200
This Is My Rifle... critical hits with assault rifles. 25 100 400 1000 2000
...This Is My Gun
(Unlocked by completing This Is My Rifle... Level 1)
critical hits using assault rifles. 10 25 75 150 300
From My Cold, Dead Hands Get Second Winds with assault rifles. 5 15 30 50 75
SMG
Hail of Bullets Kill enemies with SMGs. 25 100 400 1000 2000
More Like Submachine FUN Get Second Winds with SMGs. 2 5 15 30 50
Constructive Criticism critical hits with SMGs. 25 100 400 1000 2000
High Rate of Ire critical hits using SMGs. 10 25 75 150 300
Shotgun
Shotgun Surgeon critical hits using shotguns. 10 50 100 250 500
Shotgun! Kill enemies with shotguns. 25 100 400 1000 2000
Faceful of Buckshot critical hits with shotguns. 50 250 1000 2500 5000
Lock, Stock, and... Get Second Winds with shotguns. 2 5 15 30 50
Open Wide! Kill enemies from point-blank range with shotguns. 10 25 150 300 750
Shotgun Sniper
(Unlocked by completing Open Wide! Level 5)
Kill enemies from long range with shotguns. 10 25 75 150 300
Pistol
The Killer Kill enemies with pistols. 25 100 400 1000 2000
Quickdraw
(Unlocked by completing The Killer Level 1)
Kill enemies shortly after entering iron sights with pistols. 10 25 150 300 750
Deadeye critical hits with pistols. 25 100 400 1000 2000
Pistolero
(Unlocked by completing Deadeye Level 1)
critical hits using pistols. 10 25 75 150 300
Hard Boiled Get Second Winds with pistols. 2 5 15 30 50
Melee
Fisticuffs! Kill enemies with melee attacks. 25 100 400 1000 2000
A Squall of Violence
(Unlocked by completing Fisticuffs! Level 1)
Kill enemies with melee attacks using bladed guns. 20 75 250 600 1000
General Combat
Knee-Deep in Brass Fire a lot of rounds. 1k 5k 10k 25k 50k
Critical Acclaim critical hits. And rainbows! 20 100 500 1000 1500
...I Got to Boogie Kill enemies at night. 10 25 150 300 750
Afternoon Delight Kill enemies during the day. 50 250 1000 2500 5000
Not Full of Monkeys Kill enemies with stationary barrels. 10 25 45 70 100
...To Pay the Bills Kill enemies while using your Action Skill. 20 75 250 600 1000
Boomerbang Kill enemies using Tediore reloads. 5 10 50 100 200
Gun Slinger Deal damage with Tediore reloads. 5k 20k 100k 500k 1M
Miscellaneous
Haters Gonna Hate Win duels. 1 5 15 30 50
Yo Dawg I Herd You Like Challenges Complete many, many challenges. 5 25 50 100 200
JEEEEENKINSSSSSS!!! Find and eliminate Jimmy Jenkins. 1 3 6 10 15
Compl33tionist Complete optional mission objectives. 10 25 45 70 100
Sidejacked Complete side missions. 5 15 30 55 90

Captain Scarlett and Her Pirate's Booty DLC Challenges

Challenge Objective Number required for Level
1 2 3 4 5
In The Pink Seraph Crystals. 80 160 240 320 400
Worm Killer sand worms. 10 20 30 50 80
Land Lubber pirates. 50 100 150 200 250
Hovernator Destroy Pirate Hovercrafts. 5 10 15 20 30
Pirate's Booty Pirate Chests. 25 75 150 250 375
Hyperius the Not-So-Invincible Hyperius by zero. 1 3 5 10 15
Master Worm Food Master Gee to his worms. 1 3 5 10 15
Shady Dealings Purchase Items with Seraph Crystals. 1 3 5 10 15

Mr Torgue's Campaign of Carnage Challenges

Challenge Objective Number required for Level
1 2 3 4 5
Torgue Tokens Acquired Acquire Torgue Tokens. 100 250 500 750 1000
Bikers Killed Bikers Killed. 50 100 150 200 250
Torgue Items Purchased Tokens. 2 5 8 12 15
Battles Completed Complete All Battles. 1 4 8 12 N/A
Pete the Invincible Defeated Pete the Invincible. 1 3 5 10 15
Bikes Destroyed Destroy Bikes. 10 20 30 50 80

Sir Hammerlock's Big Game Hunt DLC Challenges

Challenge Objective Number required for Level
1 2 3 4 5
Boroking Around Kill Boroks. 10 20 50 80 120
Voracidous the Vincible Voracidous the Invincible 1 3 5 10 15
Fan Boy fan boats. 5 10 15 20 30
Harder They Fall drifters. 5 15 30 40 50
Savage Bloody Savage Kill savages. 20 50 100 250 500
Stinging Sensation Kill scaylions. 10 20 50 80 120

Tiny Tina's Assault on Dragon Keep DLC Challenges

Challenge Objective Number required for Level
1 2 3 4 5
Fus Roh Die Kill dragons. 10 20 50 80 110
Scot-Free dwarves. 50 100 150 200 250
Rock Out With Your Rock Out Kill golems. 10 25 50 80 120
Knighty Knight knights. 10 25 75 120 175
Can't Fool Me Kill mimics. 5 15 30 50 75
Orcs Should Perish orcs. 50 100 150 200 250
Bone Breaker skeletons. 50 100 150 200 250
Ew Ew Ew Ew Kill spiders. 25 50 100 150 200
Cheerful Green Giants Kill treants. 10 20 50 80 120
Magical Massacre wizards. 10 20 50 80 120