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Nitrus Brio, Dr. Neo Cortex's assistant is the penultimate boss of the game and a formidable opponent, with a very long life bar. Nitrus Brio will throw red and green explosive vials at you. The red ones explode on contact and should be avoided, while the green ones give life to bouncing jellies, which you can eliminate with a jump or a twist attack. By defeating the jellies, you will reduce the boss's life bar. When he has only three life points left, Nitrus Brio will transform into a giant and will come at you furiously, hitting the floor. At this point, you will have to use the stones that will fall from the ceiling to jump on the boss's head and damage him. Repeat the operation until you finally defeat him.