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Toadsworth is not a playable character; he just drives the Parade Kart at the award ceremony. Items in red must be unlocked first.
| Character | Weight | Special Item | Kart |
|---|---|---|---|
| Baby Luigi | Lightweight | Chain Chomp | Rattle Buggy |
| Baby Mario | Lightweight | Chain Chomp | Goo-Goo Buggy |
| Birdo | Middleweight | Birdo's Egg | Turbo Birdo |
| Bowser | Heavyweight | Bowser's Shell | Koopa King |
| Bowser Jr. | Lightweight | Bowser's Shell | Bullet Blaster |
| Daisy | Middleweight | Heart | Bloom Coach |
| Diddy Kong | Lightweight | Giant Banana | Barrel Train |
| Donkey Kong | Heavyweight | Giant Banana | DK Jumbo |
| King Boo | Heavyweight | Random Item | Boo Pipes |
| Koopa Troopa | Lightweight | Triple Shells(Red/Green) | Koopa Dasher |
| Luigi | Middleweight | Green Fireball | Green Fire |
| Mario | Middleweight | Red Fireball | Red Fire |
| Paratroopa | Lightweight | Triple Shells(Red/Green) | Para Wing |
| Peach | Middleweight | Heart | Heart Coach |
| Petey Piranha | Heavyweight | Random Item | Piranha Pipes |
| Toad | Lightweight | Golden Mushroom | Toad Kart |
| Toadette | Lightweight | Golden Mushroom | Toadette Kart |
| Toadsworth | Lightweight | No Special Item | Parade Kart |
| Waluigi | Middleweight | Bob-omb | Waluigi Racer |
| Wario | Heavyweight | Bob-omb | Wario Car |
| Yoshi | Middleweight | Yoshi's Egg | Turbo Yoshi |