Kirby
- Series: Kirby series
- Original Game: Kirby's Dream Land (Game Boy) - 1992
Link
- Series: The Legend of Zelda series
- Original Game: Legend of Zelda (NES) - 1986
Mario
- Series: Super Mario series
- Original Game: Donkey Kong (Arcade) - 1981
Meta Knight
- Series: Kirby series
- Original Game: Kirby's Adventure (NES) - 1993
Pikachu
- Series: Pokémon series
- Original Game: Pokémon Red and Blue (Game Boy) - 1996
Pit
- Series: Kid Icarus series
- Original Game: Kid Icarus (NES) - 1992
Pit was originally going to be in the first SSB but was replaced with Jigglypuff because she was thought to be more profitable. Pit fights using his bow, which can be splits into two knife type weapons. He is agile and can jump multiple times, similar to Kirby and Jigglypuff.
Samus Aran
- Series: Metroid series
- Original Game: Metroid (NES) - 1986
Solid Snake
- Series: Metal Gear series
- Original Game: Metal Gear (MSX2/NES) - 1987
Solid Snake makes an unexpected appearance in Brawl, being the first third-party character to ever debut in a Super Smash Bros. game. In Brawl, Snake does not use real-world guns, but still utilizes explosives.
Wario
- Series: Wario series
- Original Game: Super Mario Land 2: 6 Golden Coins (Game Boy) - 1992
Brawl is Wario's first debut in the world of Smash Bros. He wears the costume from the WarioWare games, as opposed to his traditional outfit. Nintendo stated that they aimed to give Wario some rather excessive movements.
Fox McCloud
- Series: Star Fox series
- Original Game: Star Fox (SNES) - 1993
Since the lift off from Super Smash Bros., Fox McCloud makes his continuity consistent in Brawl. Though not much is currently known, it has been observed that Fox will have his laser gun in hand at all times, unlike earlier in the series where he only uses his laser gun upon invocation.